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Developer's Library Software Version 2.1
Jun 2006
PicoQuant GmbHRequest Info
For NanoHarp 250's PCI Board Used in Multichannel Scaling, Photon Counting
BERLIN, June 22, 2006 -- A new Developer's Library software release is available from PicoQuant GmbH for the NanoHarp 250 data acquisition board to be used in multichannel scaling and photon counting.

The NanoHarp 250 is a short PCI slot card with integrated input discriminators and is an ideal instrument for the acquisition of fluorescence/luminescence decays on the nanosecond to millisecond time scale, PicoQuant said. Other applications include optical time-domain reflectometry (OTDR), time-of-flight mass spectrometry and range finding.

The new Developer's Library software version 2.1 now extends the capabilities of the board by making the programmable trigger output more flexible. Excitation sources such as the PDL 800-B diode laser family can now be triggered only if a measurement is running to avoid bleaching of fluorophores.

The software runs on all recent Windows platforms, including Windows ME, 2000 and XP. Data can be collected and visualized online. An intuitive and clean graphical user interface with PicoquantNanoharp.jpgcomprehensive online help guarantees a short learning curve. The new software also supports control for an extended range of monochromators, permitting the automated recording of time-resolved emission spectra. Fluorescence decay analysis is facilitated by direct transfer to the popular FluoFit software. A new programming library, including a rich set of example programs, is separately available to support the development of custom software and to aid integration with existing automated instruments, e.g. under LabView, PicoQuant said.

By means of reconfiguration of the board's programmable logic, the software provides two different measurement modes. For high resolution measurements, the fast mode provides a time bin resolution of 4 ns. In slow mode, the time bin width is 32 ns. This extends the usable time span of the decay or OTDR measurement to over two seconds. In both modes, up to 262144 time bins are available.

The board's multistop capability allows efficient recording of long-lived fluorescence decays with correspondingly slow excitation rates, the company said. On-board histogram memory is 18-bits deep, allowing the collection of 262144 counts per bin without software intervention. Software adjustable discriminators and on-board polarity switches allow the NanoHarp 250 to be interfaced to a wide range of signal and trigger sources, including all common single-photon detectors such as photomultiplier tubes and single-photon avalanche photodiodes. Count rates up to 50 MHz can be processed; histogram data is efficiently transferred by means of bus-mastering DMA.

PicoQuant develops electro-optical instrumentation and systems, picosecond diode lasers and time-resolved fluorescence systems. For more information, visit: ; e-mail:

PicoQuant GmbH
Rudower Chaussee 29
12489 Berlin, Germany
Phone: (49-30) 6392-6560
Fax: (49-30) 6392-6561


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The emission of light or other electromagnetic radiation of longer wavelengths by a substance as a result of the absorption of some other radiation of shorter wavelengths, provided the emission continues only as long as the stimulus producing it is maintained. In other words, fluorescence is the luminescence that persists for less than about 10-8 s after excitation.
A graphic representation of a distribution function such as frequency by means of rectangles whose widths represent the intervals into which the observed values' range is divided and whose heights represent the number of observations occurring in each interval.
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