A multibeam laser confocal scanner with software-selectable 10- to 64-μm-diameter pinholes has been introduced by VisiTech International. The VT-Infinity3 performs real-time confocal imaging at speeds of up to 1000 fps, which, coupled with the 3D-RT module, supports on-demand 3-D reconstruction of experimental data as it is acquired. The system is self-aligning, and the motorized dichroics and filters can be exchanged on-site. It operates from the near-UV to the near-IR and is compatible with upright and inverted microscopes. Low photobleaching permits long-duration experiments of photosensitive samples, with minimal phototoxic effects.