The Luminoview LV200 bioluminescence microscope from Olympus Life Science Europa GmbH performs imaging for small organisms and slice cultures as well as at the single-cell scale. It features full environmental control for long-term live-cell imaging, including independent temperature control for the stage, incubation chamber, top cover and objective, resulting in high sensitivity and resolution at various magnifications. Its specialized optical design maximizes light collection, enables dual-color luminescence and provides bright-field and fluorescence overlays. The device is integrated with the company’s cell^M imaging and analysis software. Applications include long-term quantitative analysis of gene expression in living cells with a high signal-to-noise ratio and spatial resolution.