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  • Phosphorescence Imaging

Feb 2010
Lambert Instruments BVRequest Info
Lambert Instruments has added the LIFA-X to its line of fluorescence lifetime imaging attachments. Suitable for long-lifetime imaging microscopy from 0.1 ns to 1 ms, it can be used for measuring reactive oxygen species in cells or oxygen concentrations using optodes. It can be attached to any wide-field fluorescence microscope and works in the homodyne frequency domain. Its high-frequency mode provides lifetime measurements in the 0 to 300-ns range, while the low-frequency mode delivers measurements in the 300-ns to 1-ms range.


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The emission of light or other electromagnetic radiation of longer wavelengths by a substance as a result of the absorption of some other radiation of shorter wavelengths, provided the emission continues only as long as the stimulus producing it is maintained. In other words, fluorescence is the luminescence that persists for less than about 10-8 s after excitation.
An instrument consisting essentially of a tube 160 mm long, with an objective lens at the distant end and an eyepiece at the near end. The objective forms a real aerial image of the object in the focal plane of the eyepiece where it is observed by the eye. The overall magnifying power is equal to the linear magnification of the objective multiplied by the magnifying power of the eyepiece. The eyepiece can be replaced by a film to photograph the primary image, or a positive or negative relay...
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