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CMOS Camera

Photonics Spectra
May 2010
Hamamatsu CorporationRequest Info
 
Hamamatsu Corp.’s ORCA-Flash2.8, a digital camera based on a scientific CMOS image sensor, is designed for low-light imaging at high frame rates. The FL-280 sensor features 2.8 megapixels and a pixel size of 3.63 x 3.63 μm. Wavelength sensitivity ranges from the UV to the visible, with peak sensitivity and >60% quantum efficiency at ~450 to 500 nm. The camera’s readout speed ranges from 45 fps at full resolution to 1273 fps with subarray readout. Features include external trigger, real-time corrections and analog gain. The camera is designed for quantitative measurements with 12-bit output, and it interfaces with a PC via the included Camera Link frame grabber. Applications include ratio imaging, Förster resonance energy transfer, fluorescence in situ hybridization and total internal reflection fluorescence microscopy, real-time confocal microscopy, semiconductor inspection and industrial imaging.


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GLOSSARY
camera
A light-tight box that receives light from an object or scene and focuses it to form an image on a light-sensitive material or a detector. The camera generally contains a lens of variable aperture and a shutter of variable speed to precisely control the exposure. In an electronic imaging system, the camera does not use chemical means to store the image, but takes advantage of the sensitivity of various detectors to different bands of the electromagnetic spectrum. These sensors are transducers...
digital camera
A camera that converts a collected image into pixels that are black or white digital or shades of gray. The digital data may then be manipulated to enhance or otherwise modify the resulting viewed image.
fluorescence
The emission of light or other electromagnetic radiation of longer wavelengths by a substance as a result of the absorption of some other radiation of shorter wavelengths, provided the emission continues only as long as the stimulus producing it is maintained. In other words, fluorescence is the luminescence that persists for less than about 10-8 s after excitation.
fluorescence microscopy
Observation of samples using excitation produced fluorescence. A sample is placed within the excitation laser and the plane of observation is scanned. Emitted photons from the sample are filtered by a long pass dichroic optic and are detected and recorded for digital image reproduction. 
frame grabber
Image processing peripheral that converts video images from cameras into digital format and transfers these digital images to PCs.
resolution
1. In optics, the ability of a lens system to reproduce the points, lines and surfaces in an object as separate entities in the image. 2. The minimum adjustment increment effectively achievable by a positioning mechanism. 3. In image processing, the accuracy with which brightness, spatial parameters and frame rate are divided into discrete levels.
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