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  • LED Illumination System

Jul 2010
Carl Zeiss Microscopy LLCRequest Info
For wide-field fluorescence microscopy applications, Carl Zeiss MicroImaging Inc. has incorporated into its Colibri illumination system high-intensity LEDs in a variety of colors that match the excitation bandwidth of many commonly used fluorescent dyes and proteins. The modular light source system uses specific wavelength windows and up to four LEDs, each individually and instantly controlled by electrical current without mechanical switching devices like filter wheels or shutters. LEDs of different colors can be used in combination, giving users the option of seeing multiple fluorochromes simultaneously or rapidly capturing sequential images of each fluorochrome. For applications requiring higher illumination intensity, or for applications requiring excitation wavelengths not currently supported by existing LED technology, the company offers a combination system that pairs a Colibri with an externally coupled metal halide white-light source.


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1. The process by which an atom acquires energy sufficient to raise it to a quantum state higher than its ground state. 2. More specifically with respect to lasers, the process by which the material in the laser cavity is stimulated by light or other means, so that atoms are converted to a semistable state, initiating the lasing process.
filter wheel
A device that holds a number of filters and allows the filter with the desired characteristics to be rotated into an optical aperture.
The emission of light or other electromagnetic radiation of longer wavelengths by a substance as a result of the absorption of some other radiation of shorter wavelengths, provided the emission continues only as long as the stimulus producing it is maintained. In other words, fluorescence is the luminescence that persists for less than about 10-8 s after excitation.
fluorescence microscopy
Observation of samples using excitation produced fluorescence. A sample is placed within the excitation laser and the plane of observation is scanned. Emitted photons from the sample are filtered by a long pass dichroic optic and are detected and recorded for digital image reproduction. 
The combination of the organic dye in a stained specimen and the antibodies produced that is detected by exposure to light.
A mechanical or electronic device used to control the amount of time that a light-sensitive material is exposed to radiation.
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