SURREY, British Columbia, July 22, 2010 — For high-quality, high-sensitivity imaging in low-light conditions, QImaging has released the Rolera EM-C2 electron-multiplying CCD (EMCCD) microscopy camera. It aids researchers in applications such as spinning-disc confocal imaging, total internal reflection fluorescence microscopy, ratiometric ion imaging, and fluorescence recovery after photobleaching. Fast frame rates essential to cutting-edge live-cell imaging studies are enabled by the camera’s 40-MHz pixel clock rate. Up to 34.2 full-resolution (one megapixel) frames per second can be read out and transferred over an optimized 800-Mb/s implementation of the IEEE 1394b FireWire protocol. The camera delivers low noise and ~65% peak quantum efficiency across a broad spectrum common to fluorescence experiments. Peltier cooling to -50 ºC minimizes dark current for longer exposures. Variable exposure times range from 200 ms to 17.9 min. The camera’s Easy-EM mode optimizes camera electron-multiplying gain settings with a single click. Application of EM gain boosts the signal-to-noise ratio, enabling researchers to acquire clear images under low-light conditions. They no longer have to gauge sensitivity using the arbitrary sliders that are required with the use of existing cameras to estimate necessary EM gain. Applying excessive EM gain increases noise and degrades the image quality, and too little EM Gain will not effectively reduce noise. The Easy-EM function sets the camera directly to an optimal signal-to-noise setting so users can capture the best possible data for every experiment. The included QCapture Suite software can be installed and operational within minutes out of the box. The camera is backed by a two-year limited warranty.