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  • DMI6000 B Inverted Microscope
Oct 2010
Leica Microsystems GmbHRequest Info
Wetzlar, Germany. Oct. 19, 2010 — For imaging of dynamic processes in living cells, Leica Microsystems GmbH has developed the DMI6000 B inverted microscope with adaptive focus control (AFC) for use by researchers who demand consistent multidimensional imaging by a microscope that is not susceptible to sudden changes in temperature.

The fully automated adaptive focus control is based on the reflection of a light beam at a suitable surface, such as a petri dish, to keep the distance between the objective and the specimen constant. Available for both wide-field (including total internal reflection fluorescence) and confocal applications, AFC dynamically regulates the focus position, whenever or wherever the experiment requires it. For more flexibility, AFC can also be operated in stand-alone mode and requires neither a PC connection nor additional external control devices.

The underlying technique works for all selected contrasting methods and does not involve the acquisition of images. This ensures that cells remain viable for longer, delivering reliable results even over extended periods of time. The intelligent automation of the system provides ease of use, automatically optimizing all settings for each objective. With the option to effectively combine AFC with a high-speed digital auto focus, cells that change their morphology and shape during the course of the experiment are now easy to follow, and their exposure to light is minimized.

The adaptive focus control is also available for the company’s AM TIRF MC, TCS SP5 II, TCS SP5 II DS and TCS SP5 MP microscopes.


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An instrument consisting essentially of a tube 160 mm long, with an objective lens at the distant end and an eyepiece at the near end. The objective forms a real aerial image of the object in the focal plane of the eyepiece where it is observed by the eye. The overall magnifying power is equal to the linear magnification of the objective multiplied by the magnifying power of the eyepiece. The eyepiece can be replaced by a film to photograph the primary image, or a positive or negative relay...
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