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  • NS200 Nanoparticle System
Feb 2011
NanoSight Ltd.Request Info
SALISBURY, England, Feb. 11, 2011 — NanoSight Ltd. has released the latest version of its NS200 nanoparticle characterization system based on the LM20 model and using the particle-by-particle visualization and counting method, nanoparticle tracking analysis. Taking advantage of developments in hardware, the company has produced an easier-to-use instrument with a high-sensitivity camera built into the housing.

Features include optimized locators to facilitate positioning of the measurement cell, an electronic readout of the cell test temperature, and an optional blue laser to improve imaging capability, and fluorescence filters can be added when working with suitably labeled particles.

The electron-multiplying CCD camera is designed with maximal speed and sensitivity. It operates at 37 full fps collected directly through a USB2.0 interface, and the laser is triggered to reduce thermal issues with respect to the sample, restricting photobleaching during fluorescence imaging.

The rugged system requires minimal operator skills. Training is rapid, and operators can produce consistent results within a short time.

With applications from life sciences to materials sciences, the system delivers multiparameter nanoparticle analysis in a single instrument. It visualizes, measures and characterizes virtually all nanoparticles. Particle size, concentration, Zeta potential and aggregation can be analyzed, and the fluorescence mode provides differentiation of labeled particles. The system performs real-time monitoring of the subtle changes in the characteristics of particle populations with all of these analyses, confirmed by visual validation.

Nanoparticle tracking analysis detects and visualizes populations of nanoparticles in liquids down to 10 nm, dependent upon the material, and measures the size of each particle from direct observations of diffusion. This particle-by-particle method goes beyond traditional light scattering and other ensemble techniques in providing high-resolution particle size distributions. It measures concentration and validates data with information-rich video files of the particles moving under Brownian motion.

The comprehensive characterization meets the demands of complex biological systems and is widely used in development of drug delivery systems, of viral vaccines, in nanotoxicology and in biodiagnostics. Real-time data lends insight into the kinetics of protein aggregation and other time-dependent phenomena in a qualitative and quantitative manner. In biodiagnostics, the system provides detection and speciation of nanovesicles (exosomes) and microvesicles.


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Brownian motion
The behavior of microscopic solid particles suspended in a fluid, first observed by botanist Robert Brown in 1827 as a continuous random motion.
A light-tight box that receives light from an object or scene and focuses it to form an image on a light-sensitive material or a detector. The camera generally contains a lens of variable aperture and a shutter of variable speed to precisely control the exposure. In an electronic imaging system, the camera does not use chemical means to store the image, but takes advantage of the sensitivity of various detectors to different bands of the electromagnetic spectrum. These sensors are transducers...
A small object that behaves as a whole unit or entity in terms of it’s transport and it’s properties, as opposed to an individual molecule which on it’s own is not considered a nanoparticle.. Nanoparticles range between 100 and 2500 nanometers in diameter.  
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