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  • Intravital Imaging

Photonics Spectra
May 2011
FEI Munich GmbHRequest Info
The optical design of Till Photonics GmbH’s Intravital2P microscope dedicated to intravital imaging increases light collection efficiency for dual-color two-photon microscopy. The ultracompact imaging platform provides an optimized dual-emission beam path for high collection efficiency in both detection channels. The enhanced signal detection facilitates imaging at low-light levels and improves signal-to-noise ratios for dynamic measurements deep within highly scattering tissue. The proprietary Yanus scan head provides good scan quality with homogeneous two-photon excitation as well as large-area scanning. Combined with its fast scanning speeds, the microscope enables long-term in vivo imaging with reduced phototoxic effects. The voice coil-based focus drive combines excursions of up to 7.5 mm with 50-nm resolution, step response time of <10 ms and high speed for 3-D views of the sample.


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An instrument consisting essentially of a tube 160 mm long, with an objective lens at the distant end and an eyepiece at the near end. The objective forms a real aerial image of the object in the focal plane of the eyepiece where it is observed by the eye. The overall magnifying power is equal to the linear magnification of the objective multiplied by the magnifying power of the eyepiece. The eyepiece can be replaced by a film to photograph the primary image, or a positive or negative relay...
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