FLIM and FCS Upgrade Kit
Jul 2011PicoQuant GmbHRequest Info
BERLIN, July 15, 2011 — PicoQuant GmbH has extended the compatibility of its upgrade kit for laser scanning microscopes. The kit, which upgrades microscopes for fluorescence lifetime imaging (FLIM) and fluorescence correlation spectroscopy (FCS), now also supports upgrades of the Zeiss LSM780 and the Leica SP2 confocal laser scanning microscopes (CLSMs).
The kit extends the capabilities of a CLSM for time-resolved measurements with resolution down to picoseconds and allows users to perform analysis methods on this time scale. Parameter dependencies can be analyzed in a multitude of ways. The fluorescence lifetime fluctuation correlation function can be calculated on any single spot of interest, the fluorescence decay of each image pixel and detector channel can be reconstructed for FLIM, and advanced time-resolved Förster resonance energy transfer (FRET) analysis can be performed.
Applying FLIM-FRET, not only can the FRET efficiency be deduced, but also the fraction of complete FRET molecules. Two-photon excitation or state-of-the-art picosecond diode laser sources for fluorescence excitation can be applied. The fluorescence emission is guided to photon-counting detectors, such as photomultiplier tubes or single-photon avalanche photodiodes, and is finally recorded by the time-correlated single-photon counting (TCSPC) module.
The synchronization signals from the laser scanning microscope scanner are fed into the data stream recorded by the TCSPC module via the time-tagged time-resolved data acquisition mode. In this mode, each photon is recorded individually with its four specific parameters as detector channel, picosecond timing, global arrival time and pixel origin.
The FLIM and FCS upgrade kit has been improved not only for the newest CLSM types, but also for older and established models to provide high-quality customer support. It can be configured for various CLSMs, detector schemes, and excitation and emission wavelengths.