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Laser Scanning Confocal Microscope

Olympus America Inc.

CENTER VALLEY, Pa., Oct. 26, 2012 — The FluoView FV1200 confocal laser scanning imaging system designed by Olympus America Inc. enables sensitive live-cell and tissue imaging with high-speed measurement. It requires low laser power, resulting in low phototoxicity and photobleaching.

The scan head features anticorrosive silver-coated galvo mirrors that increase light throughput. The microscope offers increased excitation and emission transmission efficiencies, particularly in the near-infrared.

An optional cooled GaAsP detector minimizes electrical noise and increases quantum efficiency and signal-to-noise ratio. When the GaAsP unit is used with three conventional confocal detectors, the FV1200 can acquire up to five simultaneous fluorescent channels with its near-IR 748-nm laser diode, allowing users to image DAPI, GFP, RPF, Cy5 and Cy7 simultaneously.

The system’s multipoint mapping software delivers functional measurements of high-speed calcium fluctuations in groups of cells at speeds up to 101 Hz per field, with sequential position data output up to 50,000 Hz. The pseudo-heuristic point-scanning mode allows users to optimize their scan path as laser light moves from point to point without reducing the field of view by cropping. Each point can be expanded to an array for larger-area stimulation or detection. Precise multichannel fluorescence measurements are useful for stem cell research, patch clamping and electrophysiology, optogenetic experiments with channelrhodopsin and halorhodopsin, and studies of functional output over cells and cell networks.

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