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  • Laser Scanning Confocal Microscope
Oct 2012
Olympus Scientific Solutions Americas, Industrial MicroscopesRequest Info
CENTER VALLEY, Pa., Oct. 26, 2012 — The FluoView FV1200 confocal laser scanning imaging system designed by Olympus America Inc. enables sensitive live-cell and tissue imaging with high-speed measurement. It requires low laser power, resulting in low phototoxicity and photobleaching.

The scan head features anticorrosive silver-coated galvo mirrors that increase light throughput. The microscope offers increased excitation and emission transmission efficiencies, particularly in the near-infrared.

An optional cooled GaAsP detector minimizes electrical noise and increases quantum efficiency and signal-to-noise ratio. When the GaAsP unit is used with three conventional confocal detectors, the FV1200 can acquire up to five simultaneous fluorescent channels with its near-IR 748-nm laser diode, allowing users to image DAPI, GFP, RPF, Cy5 and Cy7 simultaneously.

The system’s multipoint mapping software delivers functional measurements of high-speed calcium fluctuations in groups of cells at speeds up to 101 Hz per field, with sequential position data output up to 50,000 Hz. The pseudo-heuristic point-scanning mode allows users to optimize their scan path as laser light moves from point to point without reducing the field of view by cropping. Each point can be expanded to an array for larger-area stimulation or detection. Precise multichannel fluorescence measurements are useful for stem cell research, patch clamping and electrophysiology, optogenetic experiments with channelrhodopsin and halorhodopsin, and studies of functional output over cells and cell networks.


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An instrument consisting essentially of a tube 160 mm long, with an objective lens at the distant end and an eyepiece at the near end. The objective forms a real aerial image of the object in the focal plane of the eyepiece where it is observed by the eye. The overall magnifying power is equal to the linear magnification of the objective multiplied by the magnifying power of the eyepiece. The eyepiece can be replaced by a film to photograph the primary image, or a positive or negative relay...
A process that helps optical fibers recover from damage induced by radiation. When silica is irradiated, bonds break and attenuation increases. Light in the fiber assists in recombining the species released by the broken bonds, decreasing attenuation.
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