Request InfoPicoQuant GmbHFluorescence Lifetime Imaging (FLIM) is a very versatile microscopy method where fluorescence lifetime information is combined with spatial localization in the sample, allowing investigating, for example, biochemical and physical processes, detecting changes in the local environment of the sample, molecular interactions, or conformational changes via Förster Resonance Energy Transfer (FRET). FLIM data acquisition is typically based on Time-Correlated Single Photon Counting (TCSPC) electronics, pulsed diode lasers, and highly sensitive single photon counting detectors. Up to now TCSPC...See full productRelated content from Photonics MediaPRODUCTSBioPhotonics Jul/Aug 2023 IssueFluorescence Lifetime Imaging Microscopy ModuleA fluorescence lifetime imaging microscopy (FLIM) module has been added to Renishaw PLC’s inVia™ confocal Raman microscope. The integration can overlay both FLIM and Raman images...Promoted Content May 2022 IssueNew Wavelengths: 473 nm, 607 nm, 698 nmOxxius’ LaserBoxx family of DPSS laser / laser diode modules offers new wavelengths not readily available to the market: 473 nm (SLM) @ 50 mW, 607 nm @ 200 mW, and 698 nm @ 100 mW. This family...Promoted Content February 2024 IssueOEM-Ready Microscope SubsystemsThe Nucleus™ microscopy platform provides a complete set of interchangeable hardware modules and software tools, making it ideal for OEMs and lab managers engaged in fluorescence cellular...BioPhotonics Sep/Oct 2023 IssueAI-Powered Microscope SoftwareLeica Microsystems’ Aivia 12 software provides AI-powered image analysis. It incorporates the Cellpose deep learning model to tackle dense neuron images, improving soma detection by as much as...ARTICLESBioPhotonics May/Jun 2023 IssueStress tests borne of mice and menMen and women respond differently to stimuli, and their hearts respond differently, too. Scientists are continuing to develop an understanding as to why these disparities in physical reactions exist....BioPhotonics Nov/Dec 2022 IssueFlow Cytometry Technique Shows Promise for Early Cancer DiagnosisA digital droplet microfluidic flow cytometry technique could enable earlier recognition of tumor development and progress, by aiding in the detection of multiple microRNAs (miRNAs) in single...Photonics.com 3/14/2023Graphene Properties Add Axial Resolution Precision to 3D ImagingResearchers at the Ludwig Maximilian University of Munich (LMU) combined pulsed-interleaved MINFLUX (pMINFLUX) nanoscopy with a newly developed method for axial resolution that exploits the special...Photonics.com 9/13/2022Intelligent Microscope Framework Captures Biological EventsBiophysicists at École Polytechnique Fédérale de Lausanne (EPFL) introduced a method to automate microscope control for imaging biological events in detail with the help of...PHOTONICS HANDBOOK ARTICLESPhotonics HandbookFluorescence Lifetime Imaging: Choosing the Best ApproachThe term fluorescence is often applied as a synonym for photoluminescence, although luminescence actually covers fluorescence and phosphorescence. Both of these terms describe the process of...Photonics HandbookSuperresolution Microscopy: An Imaging RevolutionSuperresolution optical microscopy, for which the Nobel Prize was awarded to Eric Betzig, Stefan Hell, and William Moerner in 2014, has been one of the most momentous developments in the life...Photonics HandbookDigital Cameras: Smaller Pixels for Higher ResolutionIn the search for ever-higher pixel counts for the digital still camera, it is clear that, if chip size remains constant, then “more pixels” means “smaller pixels.” The...Photonics HandbookImage Processing: Turning Digital Data into Useful InformationImages are produced by many means: cameras, x-ray machines, electron microscopes, radar and ultrasound. They are used in the entertainment, medical, scientific and business industries; for security...WHITEPAPERSPhotonics.com 10/19/2022Multiphoton Imaging of Mouse IntestineTwo-photon excited fluorescence (2PEF) and second harmonic generation (SHG) are complementary multiphoton imaging techniques for studying biological samples. Both utilise femtosecond pulsed infrared...Photonics.com 2/28/2024Multi-Bandpass Filters for FluorescenceLearn how multi-bandpass filters combined with multiple LEDs can enable versatile and compact fluorescence instrumentation.Photonics.com 8/17/2022Fluorescence, Delayed Fluorescence and Phosphorescence Spectra of a TADF Emitter Measured Using the FLS1000 with a VPL Laser and Gated PMT DetectorMaterials exhibiting thermally activated delayed fluorescence (TADF) have attracted widespread research attention as a new generation of high-efficiency emitters for use in Organic Light Emitting...Photonics.com 7/29/2022Confocal Raman and Photoluminescence Microscopy for Forensic InvestigationsForensic investigations often study extremely small samples which may need analyses by multiple techniques. Confocal Raman and Photoluminescence (PL) Microscopy are well-suited techniques for dealing...WEBINARSPhotonics.com 4/14/2016FLIM (Fluorescence Lifetime Imaging in the Frequency Domain) in a NutshellJoin us for a free webinar presented by PCO! Due to advancements in CMOS image sensor technology a new camera system, pco.flim, has been created to allow for easy and fast fluorescence (and...Photonics.com 5/19/2020Squeezing More Out of Light: Innovative Approaches to Time-Resolved Flow CytometryIn this webinar, presenter Giacomo Vacca, Ph.D., founder and president of Kinetic River, will discuss recent advances in time-resolved methods of flow cytometry and how these advances are being...Photonics.com 10/26/2022KEYNOTE: FLIM in Surgical OncologyMarcu reviews the development of clinically compatible fluorescence lifetime imaging (FLIM) technology and applications in surgical oncology. Emphasis is placed on the integration of FLIM in surgical...Photonics.com 11/20/2019Frequency-Domain Fluorescence Lifetime Imaging: System Improvements and ApplicationsSince the introduction of the first frequency-domain fluorescence lifetime imaging microscopy (FD-FLIM) pco.flim camera in 2014, this camera system has been applied to many different areas, from FRET...PHOTONICS DICTIONARY+ TERMSPhotonics Dictionarysingle-photon avalanche diodeA single-photon avalanche diode (SPAD) is a specialized type of photodetector designed to detect extremely weak optical signals, down to the level of single photons. SPADs are widely used in...Photonics Dictionarytime-correlated single photon countingTime-correlated single photon counting (TCSPC) is a technique used in photon counting applications, particularly in the field of experimental physics, biophysics, and fluorescence lifetime imaging...Photonics Dictionaryelectron multiplication charge-coupled device cameraAn EMCCD (electron-multiplying charge-coupled device) camera is a type of scientific camera specifically designed for low-light imaging applications that require high sensitivity and fast readout...Photonics Dictionarylight sheet fluorescence microscopyAlso known as single plane illumination microscopy (SPIM), this process was designed for imaging of sensitive samples and fast biological processes in vivo. In this method, a light sheet illuminates...VIDEOSPhotonics.com 10/12/2021pco - Frequency Domain Fluorescence Lifetime Imaging Microscopy - A Hands-On SessionThis hands-on session about FD-FLIM by Gerhard Holst from PCO AG was given as part of the FLIM Symposium "Beginners Guide to Fluorescence Lifetime Imaging in Life Sciences".Photonics.com 1/18/2018Supporting the Photonics Industry from the Laboratory - LIGHT MATTERS 01.2018On this month's episode of Light Matters, we take you to central Massachusetts as Worcester Polytechnic Institute and Quinsigamond Community College take on a comprehensive joint photonics laboratory...Photonics.com 1/18/2018Light Matters Extra: Taking Photonics Innovations from the Lab to the Operating Room - 01.2018In this month's Light Matters Extra, we give you an in-depth look at the relationship between researchers and scientists at the University of California Davis, and surgeons and clinicians at nearby...Photonics.com 11/20/2023New Dragonfly 600 Confocal Microscope System - Andor TechnologyThe Dragonfly 600 confocal microscope system delivers outstanding multi-dimensional images from subcellular (nm) to whole organism (cm), while significantly boosting productivity. Its unrivalled...PHOTONICS BUYERS' GUIDE CATEGORIESPicosecond LasersStreak CamerasA camera streak is a high-speed cine camera used to record, on a continuously moving film, very brief events such as a flash of light. The image is recorded as a continuous image, not as a series of...AutocorrelatorsAn autocorrelator is a signal-averaging device that improves signal-to-noise ratio by comparing a sampled signal with a time-delayed form of itself.Ultrafast Pulsed LasersAn ultrafast laser is a type of laser that emits extremely short pulses of light with durations typically on the order of d(10^-15 seconds) or picoseconds (10^-12 seconds). These lasers are capable...COMPANIESPhotonics Buyers' GuidePhotron USA Inc.Photron has been a global market leader in high-end, high-speed camera systems for over forty years. The company’s continuing development of new state-of-the-art products illustrates a...Photonics Buyers' GuideDK Photonics Technology LimitedDK Photonics is a leading manufacturer of innovative and high quality optical passive components. We provide complete and integrated solutions from 400~2100nm. DK Photonics produces a large array of...Photonics Buyers' GuideLaserPoint srlLaserPoint manufactures a wide portfolio of laser power and energy meters able to perform laser measurements with high accuracy. They developed the hard coatings to absorb the laser radiation which...Photonics Buyers' GuideAndor TechnologyAndor Technology Ltd is a global developer and manufacturer of high-performance scientific cameras, microscopy systems and spectrographs for academic, government, and industrial applications. Founded...