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Microscopy White Papers
Camera Systems for Deep Learning in Medical & Life Sciences
Deep learning algorithms enable a steadily growing number of new applications that were previously considered unsolvable for machines. This White Paper explains the four steps of a typical image processing process and describes the three types of vision systems for deep learning: embedded systems, PC-based systems, and FPGA frame grabber-based systems.
4 Steps to Optimize the Optics in Automated Imaging Instruments
Optimize the performance and cost of your automated optical imaging system with 6 key equations for selecting an imaging sensor, objective lens, Z-focusing nano-positioning stage and XY sample positioning motion. Topics covered include: optimal...
Industrial Equipment Failure Analysis Using FT-IR Microscopic Techniques
This work demonstrates that FT-IR microscopy analysis is capable of determining the cause of failures of many different pieces of industrial equipment. This analysis can then allow the user to take preventative action measures in order to improve...
Near-Field Scanning Optical Microscopes: Capabilities and Applications
NSOM techniques and instrumentation have evolved to become vital tools for material characterization, providing high-quality data and continually expanding utility, spot-on accuracy, adaptability, and exceptionally high definition. This article...
Atomic Force Microscopy Enhances the Nanoscopy Toolkit
Imaging and morphology represent a critical puzzle piece when trying to understand nanoscale structures, whether they are naturally occurring, such as viruses, or engineered structures, such as nano-antennas and photonic devices. In addition to...
High-Speed Imaging in Biomedical Microfluidic Research
This whitepaper discusses multiple case studies when Phantom cameras have assisted in the advancement of microfluidic and cellular research.
Understanding Virus Mechanisms – One Particle at a Time
Single-molecule microscopy techniques facilitate direct study of molecular mechanisms, enabling leaps in understanding surrounding how viruses assemble, disassemble, and interact with their hosts. In this researcher profile, we describe how Prof....
Knight Optical Meets Customer's Quality Expectations
It is with great pleasure that Knight Optical – the industry-leading supplier of metrology-tested, on-spec optical components – announces a 99.9% pass rate from its state-of-the-art Metrology Laboratory and dedicated QA department. Since 1991, we...
Recent Research Using Meadowlark Optics Spatial Light Modulators
This ever-evolving collection of recent research using Meadowlark Optics Spatial Light Modulators offers a brief overview of specific applications in which Meadowlark Optics’ Spatial Light Modulators are most commonly used. Citations to published...
Advantages of Measuring Surface Roughness with White Light Interferometry
This application note discusses the use of mean roughness measurements with white light interferometry (WLI) optical profilers. Spatial filters are explained, as well as some of the normative standards requirements from ASME B46.1-2009, ISO 13565-12...
Automation on a budget: Capturing high-speed multi-labelled events with LED illumination systems
The light source for widefield fluorescence microscopy might seem like an unlikely enabler for high-speed live cell imaging, but the level of control that comes with LED Illumination Systems has changed the game. Capturing dynamic cellular processes...
Automated Scratch-Dig Inspection - Advantages of Replacing Manual-visual Inspection
Worldwide a tendency can be observed to increase accuracy and quality of inspection of optical parts. More often than ever before, manufacturers explore computer-based inspection systems to gradually replace human inspectors. We describe how machine...
Custom vs. Off-the-Shelf Solutions: Are you dreaming big enough?
Custom positioning solutions provide more leeway for innovation, and at far less cost, than many customers realize. Irrespective of whether the goal is a one-off solution or multiple units for a wider industrial instrument, an evaluation of standard...
Are Large Image Sensors A Perfect Fit for Large Field of View Microscope Applications?
In the last years nearly all microscope manufacturers used image circles with 18 mm diameter to image their field of view to cameras connected to their microscopes. Only recently some microscope manufacturers increased their field of view to offer...
Multi-Wavelength Laser Sources for Multi-Color Fluorescence Microscopy
Multi-color fluorescence microscopy is a widely used technique to generate multi-spectral images of cells and other small objects for identifying the spatial distribution of molecules of interest in complex heterogeneous samples. In this article, we...
Laser-Based Sample Preparation for Microstructure Diagnostics
There is an increased demand for preparation techniques tuned to manifold methods of microstructure diagnostics that need to be fast, reliable, cost effective, artifact-free, and targeted. Traditional mechanical preparation and focused-ion-beam...
Close Is Not Close Enough: Micropositioners' Role In Nanopositioning
When working at the nanoscale, every input has seismic impact on the ability to view, position, or manipulate the object of their attention. Whether the application is in single-molecule spectroscopy, optical trapping, super-resolution microscopy,...
Fluorescence Microscopy Technique Provides New Views of Biological Processes
Introduction to the technique of MicroMirror TIRF (total internal reflection fluorescence) microscopy. The MicroMirror TIRF microscopy technique offers significant signal-to-noise ratio improvements compared to dichroic based TIRF microscopy when...
Camera-based Products - Benefits of an ISO13485:2016-Certified Supplier
What advantages does a supplier certified according to the medical device standard ISO 13485:2016 offer to a manufacturer of camera-based medical products? Our White Paper describes the requirements of this standard, presents various ways in which...
A High-Quality Raman Microscope Can Be Recognized by These 5 Criteria
Raman spectroscopy long remained a technique that was only performed in special laboratories. In recent years, however, it has been increasingly losing its outsider status. The reason for this is the development of the confocal Raman microscope,...
Is It True That Cooled Cameras are More Sensitive than Non-cooled Cameras?
The temperature has a significant impact on certain noise sources prevalent in cameras, which contribute to the total readout noise of an image sensor. When electronic devices are operated continuously, they dissipate heat due to losses in the...
Behind the Scenes of Today's Imaging Process
Image processing has become so ingrained in everyday life that we often forget how quickly it has advanced over the last few decades. Today, we are able to snap photos and then rotate, crop, and apply various filters to the images directly on the...
Time-resolved Fluorescence Spectroscopy and Microscopy in Materials Science
Studying luminescence lifetime data is a very powerful analytical tool to gain insights into the excited state dynamics of molecules, nanoparticles, or semiconductors. Time-resolved methods such as Fluorescence Lifetime Imaging are commonly used in...
Advantages of Optically Pumped Semiconductor Lasers – Invariant Beam Properties
The optically pumped semiconductor laser (OPSL) is a unique patented technology that combines the most desirable attributes of laser diodes, DPSS (Diode Pumped Solid State) and Ion lasers, while eliminating a number of their compromising...
Hyperspectral Imaging Cameras in Rapid Detection and Potential Diagnosis of Agricultural Crop Threats
Remote monitoring of crops via satellites using color cameras was the earliest form of spectral imaging in agricultural management. Due to limited capabilities, only basic plant health parameters could be reported. The advent of hyperspectral...
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May 2024
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