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fluoresce Dictionary Terms

monochromator
A monochromator is an optical instrument used to isolate and select a narrow range of wavelengths from a broader spectrum of light. The term monochromator is derived from "monochromatic," meaning consisting of a single color or wavelength....
ratiometry
Ratiometry is a technique used in various scientific fields, particularly in analytical chemistry and biochemistry, to...
laser-induced fluorescence
Laser-induced fluorescence (LIF) is a spectroscopic technique that involves using a laser to excite molecules or atoms,...
Förster resonance energy transfer
Förster resonance energy transfer (FRET) is a mechanism describing the transfer of energy between two closely spaced...
atomic fluorescence spectroscopy
The analysis of fluorescence emitted by discrete atoms, in flames, that have absorbed radiation from an external source. It...
immunofluorescence
The technique that uses light to detect and analyze the antibodies produced by a specimen stained with an organic dye.
spectroscopy
Spectroscopy is a technique used in the study of interactions between matter and electromagnetic radiation. It involves...
cine fluorography
The application of a cine camera in recording the images on a fluorescent screen. When x-rays are used to produce the screen...
dichroic mirror
A dichroic mirror, also known as a dichroic beamsplitter or interference filter beamsplitter, is an optical device that...
single-molecule FRET
Single-molecule Förster resonance energy transfer (smFRET) is a specialized technique in biophysics and molecular...
actinometer
A device that measures the intensity of photochemically active radiation, particularly from the sun. One form of this...
infrared photomicrography
Photographic recording that uses infrared radiation as the light source to form an image of a microscopic object and a...
three-level laser
A laser having a material, such as ruby, that has an energy state structure of three levels: the ground state (1) wherein...
beta fluorography
The use of a short-duration electron beam to record high-speed events that occur in microscopic objects made of materials...
scintillation camera
A pinhole camera used to record a radioactive tracer's distribution in a subject by means of a scintillation counter or a...
fiber photometry
An optical recording technique that uses light source(s), a beamsplitting cube, light detector(s) and an optical fiber...
glass dosimeter
A device that detects and measures the quantity of exposure to nuclear radiation. It uses a special glass rod that...
fluorescence lifetime
Fluorescence lifetime refers to the average time it takes for a fluorophore, a molecule that absorbs light at one wavelength...
kinescope
A cathode-ray tube that serves as a picture tube in a television receiver. The signal representing the picture intensity is...
fluorescence quenching
The suppression of fluorescence by absorption of the stimulating radiation.
phosphor thermometry
A method for remote measurement of the temperature of moving surfaces in harsh environments by using a laser to stimulate...
nonradiative transition
A nonradiative transition refers to a process in which an electron or an atom undergoes a change in its energy state without...
spatially offset Raman spectroscopy
Spatially offset Raman spectroscopy (SORS) is a technique that extends traditional Raman spectroscopy by incorporating...
sensitized fluorescence
The optical energy transfer between ions of differing atoms.
resonance spectrum
The fluorescent emission spectrum emitted by certain substances following the irradiation of one of these substances with...
wavelength shifter
A photofluorescent compound that, when used with a scintillating substance, absorbs photons and emits related photons having...
flame spectroscopy
The study of flames by means of a laser emitting blue light and a spectrometer to measure the green fluorescence created by...
laser-excited Shpol'skii spectroscopy
A type of fluorescence spectroscopy in which an excimer laser, a dye laser and a frequency-doubling device are used to...
microfluoroscope
A fluoroscope equipped with a magnified, fine-grained fluorescent screen to determine the fluorescence emitted by a...
long-pass filter
A long-pass filter is an optical filter that allows longer wavelengths of light to pass through while blocking or...
fluorescence
Fluorescence is a type of luminescence, which is the emission of light by a substance that has absorbed light or other...
allochromy
Any fluorescence in which the wavelength of the emitted light differs from that of the absorbed light.
photomultiplier tube
A photomultiplier tube (PMT) is a highly sensitive vacuum tube that detects and amplifies low levels of light. It is widely...
fluorographic lens
A lens having an extremely high aperture and used in the recording of x-ray fluorescent screen images. It often is specially...
fluoroscope
An instrument used to detect the fluorescence emitted by a source in comparison with a reference.
medical lasers
Medical lasers are devices that produce intense beams of light with specific characteristics and properties, which are used...
electron multiplication charge-coupled device camera
An EMCCD (electron-multiplying charge-coupled device) camera is a type of scientific camera specifically designed for...
cadmium sulfide
An inorganic compound, yellow to orange in color, that fluoresces strongly enough when bombarded by a high-current-density...
x-ray detector
One of various types of fluorescent screens used to detect x-ray radiation. Photographic film is mildly sensitive to x-rays,...
two-photon fluorescence
This results from the simultaneous absorption of two photons, each having half the energy needed for excitation and...
fluorescent whitening agents
Agents used for testing light sources and natural daylight with visual and instrumental assessment using a set of white...
single-photon avalanche diode
A single-photon avalanche diode (SPAD) is a specialized type of photodetector designed to detect extremely weak optical...
single-walled carbon nanotubes
Referred to as SWCNTs, these cylindrical nanostructures composed of a folded sheet of graphene can be used as near-infrared...
intensified charge-coupled device camera
An intensified charge-coupled device (ICCD) camera is an advanced imaging device that combines the capabilities of both an...
resonance fluorescence
In atomic fluorescence spectroscopy, fluorescence emitted by an atom at a spectral line of a wavelength identical to that of...
phosphor
A chemical substance that exhibits fluorescence when excited by ultraviolet radiation, x-rays or an electron beam. The...
wide-field fluorescence microscopy
Wide-field fluorescence microscopy uses either naturally occurring structures or staining with fluorescent tags that are...
superresolution
Superresolution refers to the enhancement or improvement of the spatial resolution beyond the conventional limits imposed by...
fluorescent screen
A fluorescent screen refers to a phosphorescent or fluorescent-coated surface that emits visible light when exposed to other...
fluorophore
A fluorophore is a molecule or a portion of a molecule that has the ability to emit light upon excitation by an external...
fiber optic imaging bundle
A fiber optic imaging bundle is a specialized optical device composed of multiple optical fibers bundled together. Each...
direct-line fluorescence
With respect to atomic fluorescence spectroscopy, the fluorescence that is emitted by an atom at a spectral line of a...
light sheet fluorescence microscopy
Also known as single plane illumination microscopy (SPIM), this process was designed for imaging of sensitive samples and...
scientific CMOS camera
A scientific CMOS (complementary metal-oxide-semiconductor) camera is a type of digital camera specifically designed for...
quantum dots
A quantum dot is a nanoscale semiconductor structure, typically composed of materials like cadmium selenide or indium...
optical fluorography
The fluorographic method whereby the visible image (as opposed to the x-ray image) is photographed by mounting a camera in...
cineradiography
The photographic filming of the action of x-ray images recorded on a fluorescent screen by means of large lens apertures and...
metabolic imaging
Metabolic imaging refers to the visualization and quantification of metabolic processes within living organisms, tissues, or...
laser-induced fluorescence spectroscopy
A pump-probe technique whereby a single wavelength source may be used in order to excite a given sample to determine...
luminescence
Luminescence is the emission of light that occurs without the involved substance undergoing a significant increase in...
fluorescent lamp
hematoporphyrin derivative
A material used in photodynamic therapy that is retained selectively by tumor tissue when injected into the body; it then...
impact fluorescence
Fluorescence formed when atoms of one element collide with excited atoms of another.
scientific complementary metal-oxide semiconductor
Scientific complementary metal-oxide semiconductor (sCMOS) refers to a type of image sensor technology specifically designed...
halogen lamp
A halogen lamp, also known as a tungsten halogen, quartz-halogen, or quartz iodine lamp, is an incandescent lamp that uses a...
image converter
An electron tube that employs electromagnetic radiation to produce a visual replica of an image produced on its cathode....
photon correlation spectroscopy
Spectroscopy used to study the concentration, diffusion and Brownian motion of small particles suspended in a fluid by...
epifluorescence microscopy
Fluorescence imaging technique in which the excitation light from the objective is directed into the sample producing...
fluorescence lifetime imaging
Fluorescence lifetime imaging (FLIM) is an advanced imaging technique that provides information about the lifetime of...
x-ray detection
The collection and detection of x-rays by virtue of their ionizing properties. The ionization may be perceived directly by a...
blue diode laser
A blue diode laser is a type of semiconductor laser that emits light in the blue wavelength range of the electromagnetic...
nanoparticle photonic resonator absorption microscopy
Nanoparticle photonic resonator absorption microscopy is a microscopy technique that combines the principles of photonic...
autofluorescence
Autofluorescence refers to the natural emission of fluorescence exhibited by certain biological structures or molecules when...
nitrogen vacancy
A nitrogen vacancy (NV) refers to a specific type of defect or impurity in a crystal lattice where a nitrogen atom replaces...
fluorescence photography
The photographic recording of a subject that exhibits luminescence only for an extremely short time (10-8 s) after the...
impurity level
In the context of optical materials, the term "impurity level" refers to the presence of foreign atoms or molecules within...
flow cytometry
Flow cytometry is a powerful technique used in biology and medicine for the quantitative analysis of the physical and...
fluorescent light source
A tube containing mercury vapor and lined with a phosphor. When current is passed through the vapor the strong ultraviolet...
illuminated table
A desklike apparatus with an opal glass surface illuminated from beneath by fluorescent tubes. It is equipped with roll...
optical filter
An optical filter is a device that selectively transmits or blocks specific wavelengths, or colors, of light. Optical...
electron image tube
A cathode-ray tube that increases the brightness or size of an image or forms a visible image from invisible radiation. The...
photobleaching
Photobleaching is a phenomenon in which the fluorescence of a fluorophore (a fluorescent molecule or dye) is permanently...
high-content screening
Also known as HCS, an analytical method designed to collect statistically relevant amounts of quantitative data on many...
fluorescence microscopy
Fluorescence microscopy is a specialized optical imaging technique used in biology, chemistry, and materials science to...
time-correlated single photon counting
Time-correlated single photon counting (TCSPC) is a technique used in photon counting applications, particularly in the...
structured illumination microscopy
Structured illumination microscopy (SIM) is an advanced optical imaging technique used in microscopy to enhance the...
primary fluorescence
Fluorescence produced as a result of the intrinsic property of the material itself or the doping of the material with trace...
stochastic optical reconstruction microscopy
Stochastic optical reconstruction microscopy (STORM) is a superresolution microscopy technique that enables imaging of...
photoelectric fluorometer
A filter fluorometer that uses a photomultiplier tube to detect the fluorescence of a sample.
two-photon excited fluorescence
Two-photon excited fluorescence (TPEF) is a nonlinear optical method that allows imaging of biological cells and living...
spectral flow cytometry
Spectral flow cytometry is an advanced flow cytometry technique that expands the capabilities of traditional flow cytometry...
ultraviolet fiber optics
Ultraviolet (UV) fiber optics refers to optical fibers that are designed and optimized for the transmission of ultraviolet...
optical coherence tomography angiography
Also known as OCT-A, optical coherence tomography angiography is an imaging technique that uses light waves to measure...
time-to-amplitude converter
A time-to-amplitude converter (TAC) is an electronic circuit that converts a time interval between two events into an...
fluorescent microscope
A type of optical microscope that allows the specimen being viewed to be irradiated by ultraviolet, violet and occasionally...
electron telescope
An instrument that serves to produce an enlarged electron image on a fluorescent screen by focusing an infrared image of a...
electron micrograph
The photographic recording of images produced by the electrons from an electron microscope. The electron beam carries the...
contact fluorography
A fluorographic method whereby the sensitive photographic medium is pressed against a fluorescent screen to form a visible...
fiber optic window
The face of a cathode-ray tube (CRT) that has a fiber optic sheet attached to its surface. The sheet's fibers are at right...
single molecule localization microscopy
Single molecule localization microscopy (SMLM) is a group of super-resolution microscopy techniques that surpass the...
laser microscopy
Technique using functional optical microscope with the addition of a coherent source collinear with the image path. The...
metascope
A sensing or image-forming detector that serves to convert infrared rays into visible signals for communication purposes....
photon burst detection of fluorescence
A type of laser spectroscopy used to measure short-lived isotopes by observing sudden bursts of fluorescence resulting from...
rare-earth elements
Rare-earth elements (REEs) are a group of seventeen chemical elements found in the Earth's crust, characterized by their...
exciter filter
In ultraviolet and fluorescence photography, the term applied to the filter used in the photographic system and with the...
label-free
Label-free refers to a technique or method that does not require the use of additional labels, tags, or markers to detect or...
recombinase polymerase amplification
Recombinase polymerase amplification (RPA) is a molecular biology technique used to rapidly amplify specific DNA or RNA...
native fluorescence
The light emitted from tissues without the use of fluorescent dyes as markers. Because cancerous tissues and normal tissues...
intravital microscopy
Intravital microscopy is an imaging technique used to visualize biological processes within living organisms, typically in...
fluorographic camera
A camera with a very high aperture lens or mirror system for photographing x-ray fluorescent screen images, mainly to save...
fluorescence spectroscopy
The spectroscopic study of radiation emitted by the process of fluorescence.
total internal reflection fluorescence
Total internal reflection fluorescence (TIRF) microscopy is an advanced fluorescence microscopy technique that leverages the...
fluorescent protein
Fluorescent proteins are proteins that exhibit the property of fluorescence, which is the ability to absorb light at a...
photodetector
A photodetector, also known as a photosensor or photodiode, is a device that detects and converts light into an electrical...
secondary fluorescence
Fluorescence produced by a material that has been treated with a dilute solution of fluorescing material.
CMOS quantitative polymerase chain reaction system
This type of miniaturized system, referred to as CMOS qPCR, combines microfluidic technology with an ultralow-light CMOS...
APD detector
An APD detector, or avalanche photodiode detector, is a type of photodetector used to detect light or optical signals by...
fluorometer
An instrument used to measure the duration of fluorescence emanating from a biological sample to monitor and evaluate its...
microscopy
Microscopy is a scientific technique that involves the use of microscopes to observe and study objects that are too small to...
image tube camera
A camera system in which the image formed on the fluorescent screen of an image converter tube in the system is recorded by...
lab-on-a-chip
A lab-on-a-chip (LOC) is a miniaturized device that integrates various laboratory functions and capabilities onto a single,...
superfluorescence
The process in which the normal rate of fluorescent emission from a substance is enhanced by virtue of the optical gain of...
fluorometry
The analysis and measurement of the fluorescence emitted by a source. Fluorometric processes are more sensitive than light...
spectrofluorometer
An automatic scanning instrument that is used to study a substance's fluorescence over a wide range of wavelengths. It...
daylight lamp
An incandescent or fluorescent lamp whose emittance spectrum resembles that of daylight.
halation
1. In a cathode-ray tube, the glow surrounding a bright spot that appears on the fluorescent screen as the result of the...
calcium indicator
A calcium indicator is a molecule that is sensitive to changes in calcium ion (Ca2+) concentrations in biological systems....
STED microscopy
STED microscopy, or stimulated emission depletion microscopy, is a superresolution imaging technique in fluorescence...
fluorescence decay system
A device, also called a fluorescence lifetime instrument, that measures decay curves of fluorescing samples. It generally...
field ion microscope
An extremely powerful microscope that renders individual ionized atoms visible by using an electric field to propel the ions...
cathode-ray tube
A vacuum tube with an electron gun at one end and a fluorescent screen at the other. Electrons emitted from a heated...
three-photon microscopy
Three-photon microscopy is an advanced imaging technique that belongs to the family of multi-photon microscopy methods. It...
fiber optic spectrometer
A fiber optic spectrometer is a device used for measuring the spectral content of light. It utilizes optical fibers to...
fluorography
The photographic recording of a visible image formed by the impact of invisible radiation on a fluorescent screen.
fluorescence correlation spectroscopy
A powerful method, referred to as FCS, for determining the average diffusion coefficients of fluorescent molecules in...

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