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fluorescence microscopy Dictionary Terms

photobleaching
Photobleaching is a phenomenon in which the fluorescence of a fluorophore (a fluorescent molecule or dye) is permanently reduced or eliminated upon prolonged exposure to light. This process occurs due to the photochemical destruction or alteration...
autofluorescence
Autofluorescence refers to the natural emission of fluorescence exhibited by certain biological structures or molecules when...
fluorescent screen
A fluorescent screen refers to a phosphorescent or fluorescent-coated surface that emits visible light when exposed to other...
fluorescence microscopy
Fluorescence microscopy is a specialized optical imaging technique used in biology, chemistry, and materials science to...
microscopy
Microscopy is a scientific technique that involves the use of microscopes to observe and study objects that are too small to...
intensified charge-coupled device camera
An intensified charge-coupled device (ICCD) camera is an advanced imaging device that combines the capabilities of both an...
fluorescence lifetime imaging
Fluorescence lifetime imaging (FLIM) is an advanced imaging technique that provides information about the lifetime of...
total internal reflection fluorescence
Total internal reflection fluorescence (TIRF) microscopy is an advanced fluorescence microscopy technique that leverages the...
three-photon microscopy
Three-photon microscopy is an advanced imaging technique that belongs to the family of multi-photon microscopy methods. It...
fluorophore
A fluorophore is a molecule or a portion of a molecule that has the ability to emit light upon excitation by an external...
electron multiplication charge-coupled device camera
An EMCCD (electron-multiplying charge-coupled device) camera is a type of scientific camera specifically designed for...
ultraviolet fiber optics
Ultraviolet (UV) fiber optics refers to optical fibers that are designed and optimized for the transmission of ultraviolet...
fluorescence
Fluorescence is a type of luminescence, which is the emission of light by a substance that has absorbed light or other...
two-photon excited fluorescence
Two-photon excited fluorescence (TPEF) is a nonlinear optical method that allows imaging of biological cells and living...
wide-field fluorescence microscopy
Wide-field fluorescence microscopy uses either naturally occurring structures or staining with fluorescent tags that are...
STED microscopy
STED microscopy, or stimulated emission depletion microscopy, is a superresolution imaging technique in fluorescence...
Förster resonance energy transfer
Förster resonance energy transfer (FRET) is a mechanism describing the transfer of energy between two closely spaced...
long-pass filter
A long-pass filter is an optical filter that allows longer wavelengths of light to pass through while blocking or...
dichroic mirror
A dichroic mirror, also known as a dichroic beamsplitter or interference filter beamsplitter, is an optical device that...
fluorescent protein
Fluorescent proteins are proteins that exhibit the property of fluorescence, which is the ability to absorb light at a...
calcium indicator
A calcium indicator is a molecule that is sensitive to changes in calcium ion (Ca2+) concentrations in biological systems....
blue diode laser
A blue diode laser is a type of semiconductor laser that emits light in the blue wavelength range of the electromagnetic...
optical filter
An optical filter is a device that selectively transmits or blocks specific wavelengths, or colors, of light. Optical...
nanoparticle photonic resonator absorption microscopy
Nanoparticle photonic resonator absorption microscopy is a microscopy technique that combines the principles of photonic...
light sheet fluorescence microscopy
Also known as single plane illumination microscopy (SPIM), this process was designed for imaging of sensitive samples and...
epifluorescence microscopy
Fluorescence imaging technique in which the excitation light from the objective is directed into the sample producing...
scientific complementary metal-oxide semiconductor
Scientific complementary metal-oxide semiconductor (sCMOS) refers to a type of image sensor technology specifically designed...

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