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Imaging Biology at High Spatiotemporal Resolution

Oct 29, 2014
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ABOUT THIS WEBINAR
Join us for a FREE webinar Wednesday, Oct. 29, 2014, 1 p.m. EST

Presenter Dr. Hari Shroff will discuss efforts to develop high-resolution optical methods better suited than conventional imaging tools for the study of live, dynamic and 3-D biological samples. Structured illumination microscopy (SIM) doubles the spatial resolution of a light microscope, and requires lower light intensities and acquisition times than other superresolution techniques, but has been mostly applied to the study of single cells.

Shroff will present alternative SIM implementations that permit resolution doubling in live volumes greater than 10 times thicker than possible with conventional SIM, as well as a hardware modification that enables effectively "instant" SIM imaging at rates 10 to 100 times faster than other SIM implementations.

The second half of the talk will focus on the development of inverted selective plane illumination microscopy (iSPIM), and subsequent application to the noninvasive study of neurodevelopment in nematode embryos. He will discuss progress that quadruples the axial resolution of iSPIM by utilizing a second specimen view, thus enabling imaging with isotropic spatial resolution (dual-view iSPIM, or diSPIM). Shroff will conclude by introducing a protocol for a do-it-yourself diSPIM.

Dr. Hari Shroff received a bachelor's degree in bioengineering from the University of Washington in 2001, and under the supervision of Dr. Jan Liphardt, completed his doctorate in biophysics at the University of California, Berkeley, in 2006. He spent the next three years performing postdoctoral research under the mentorship of Nobel laureate Eric Betzig at the Howard Hughes Medical Institute's Janelia Farm Research Campus, where his research focused on development of photoactivated localization microscopy (PALM), an optical superresolution technique. Since 2009, Shroff has led the Section on High Resolution Optical Imaging at the National Institute of Biomedical Imaging and Bioengineering, where he and his staff develop new imaging tools for application in biological and clinical research.
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