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DNA sequencing by color

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Method could contribute to “thousand-dollar genome”

Gary Boas

The health care community and medical researchers would benefit from accurate and economical high-throughput DNA sequencing methods that can explore the complete human genome sequence. To this end, scientists are pursuing the development of DNA sequencing-by-synthesis methods based on polymerase reaction amplification. Researchers have explored the potential of genome sequencing via pyrosequencing — a sequencing-by-synthesis method based on detection of the pyrophosphate group generated when a nucleotide is incorporated in a DNA polymerase reaction. However, inherent difficulties with this...Read full article

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    Published: March 2007
    BiophotonicsDNAfluorescence detectionpyrosequencingResearch & Technology

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