David L. ShenkenbergVisualizing single nanoparticles and following them in three dimensions is useful for scientific applications ranging from examining biological cells to studying mixing in microfluidics. However, tracking nanoparticles in 3-D requires imaging outside of the focal plane, resulting in a loss of focus. Although setups with multiple cameras and beamsplitter mirror arrays can produce sharp 3-D images, the expense and complexity of such setups have prevented their widespread acceptance. 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