DOUGLAS FARMER, SENIOR EDITOR [email protected]Researchers from Washington University in St. Louis have found a mechanism to efficiently and accurately measure the point spread functions (PSFs) of the position of molecules, called variance upper bound (VUB). They believe this will someday aid scientists and clinicians as they look to understand what is happening at the molecular level in the body. The researchers specifically used fluorescent molecules to bind with amyloid fibers and measure their differences in structure. As it turns out, in so doing, they found a way to bypass typical limitations associated with single-molecule...Read full articleRelated content from Photonics MediaArticlesLabel-Free Imaging Shows Dynamics of Intracellular Cargo TransportUntil now, scientists have relied on fluorescence microscopy techniques to study intracellular cargo transport, a vital process to maintaining essential cellular functions. However, the effects of...BioPhotonics Jan/Feb 2024 IssueMicroscopy Method Uses Infrared Light to Image Molecules with Vibrational ContrastThe complementary strengths of fluorescence and vibrational microscopy are combined in a new technique developed at Caltech, which is called bond-selective fluorescence-detected infrared-excited...BioPhotonics Sep/Oct 2023 IssueInnovations in the STORM Method Reveal Vital Clues to Disease FormationScientists and clinicians have long believed that visualizing structural changes in the nucleus of cancer cells would unlock vital clues about how the disease first forms. This information could...BioPhotonics Nov/Dec 2022 IssuePrecision Positioning Aids Microscopes with Tracking Viral ProgressionThe rapid spread of COVID-19 and other diseases has shown that understanding viral infection is critical to the health of people around the world. In particular, understanding the dynamics of the...BioPhotonics Jul/Aug 2023 IssueProductsXYZ Nanopositioners for MicroscopyThe Nano-LPS and Nano-LPQ are ultra-low profile 3-axis nanopositioning systems with sub-nanometer precision under closed loop control. These piezo nanopositioners incorporate our exclusive PicoQ®...Promoted Content October 2022 IssueAward-winning Microscopy IlluminationCoolLED, a global expert in LED microscopy illumination, is helping scientists reach the full potential of their widefield fluorescence microscopy and optogenetics experiments with the popular pE-300...Promoted Content September 2022 IssuePowerful LED Illumination for Every ApplicationCoolLED, a global expert in LED microscopy illumination, is helping scientists reach the full potential of their widefield fluorescence microscopy and optogenetics experiments with its broad range of...Promoted Content May 2023 IssueNEW 4-Wavelength LED IlluminationCoolLED has launched its new pE-400 Series Illumination Systems where sophisticated control combines with powerful illumination for cost-effective fluorescence microscopy, from routine to advanced...Promoted Content December 2022 IssuePhotonics Handbook ArticlesLasers for Microscopy: Major TrendsLaser development for the microscopy market continues to be driven by key trends in applications, which currently include superresolution techniques, multiphoton applications in optogenetics and...Photonics HandbookMicroscopy Illumination: Considering Nonlaser Light SourcesAs recently as five years ago, shopping for a microscope light source was fairly straightforward. For routine applications, it was tungsten halogen, typically 100 W, while for fluorescence, it was an...Photonics HandbookVibration Control: Limiting Mechanical NoiseIn general, there are three common approaches to reducing mechanical excitation of photonic systems. The first is identifying the sources of vibration and implementing ways to eliminate or reduce...Photonics HandbookInterferometry: Measuring with LightAn interferometer is an instrument that compares the position or surface structure of two objects. The basic two-beam division of amplitude interferometer components consists of a light source, a...Photonics HandbookWhite PapersIs a DIY Microscope for You?For some advanced microscopy and nanoscopy techniques it becomes necessary to modify existing instrumentation or build an instrument from scratch. This article helps readers determine whether a...Photonics.com 4/20/2023FISBA READYBeam™ - Compact Powerful Laser SourceThe FISBA READYBeam™ is our answer to customers' increasing demand for compact, powerful, and reliable multicolor laser source that can be easily integrated into existing set-ups. The multicolor...Photonics.com 12/2/2022Principles of Scanning Nitrogen-Vacancy Magnetometry ExplainedScanning nitrogen-vacancy (NV) magnetometry is a measurement technique that combines scanned probe microscopy (SPM) with optically detected magnetic resonance (ODMR) to image magnetic field...Photonics.com 11/21/2022Product Development Strategies for Electro-Optical SystemsThis whitepaper looks at the unique challenges associated with optical systems by exploring two extremes, an ultra-high precision instrument, and a low-cost consumer product, and the different...Photonics.com 10/4/2022WebinarsMulti-Line Lasers or Laser Combiners: What Solution Is Best for Fluorescence Imaging?Fluorescence imaging is a key technique in both biomedical research and clinical diagnosis. Fluorescence-based microscopy for high-resolution and high-throughput multifluorophore imaging typically...Photonics.com 6/17/2021How Pixel Size and MTF Affect Modern Microscopy and See the Invisible with MicroscopesMicroscope designs are moving toward larger field-of-view and larger image circles to capture more information with the recording of a single image. For that reason, camera manufacturers are...Photonics.com 1/13/2022Probes Provide Supermultiplexed Imaging of Tissues and SystemsBiological systems with intrinsic complexity require multiplexing techniques to comprehensively describe the phenotype, interaction, and heterogeneity. However, fluorescence can only image two to...Photonics.com 10/25/2022How the Kinetix sCMOS Camera Broke the Golden Rule of Compromise in Scientific ImagingIn this webinar, product and applications experts from Teledyne Photometrics are joined by leading imaging scientists to discuss how the latest developments in scientific CMOS (sCMOS) technology are...Photonics.com 3/23/2021Photonics Dictionary TermssuperresolutionSuperresolution refers to the enhancement or improvement of the spatial resolution beyond the conventional limits imposed by the diffraction of light. In the context of imaging, it is a set of...Photonics Dictionarystochastic optical reconstruction microscopyStochastic optical reconstruction microscopy (STORM) is a superresolution microscopy technique that enables imaging of biological specimens at resolutions beyond the diffraction limit of conventional...Photonics Dictionarysingle molecule localization microscopySingle molecule localization microscopy (SMLM) is a group of super-resolution microscopy techniques that surpass the diffraction limit of traditional optical microscopes, allowing for the...Photonics Dictionaryintensified charge-coupled device cameraAn intensified charge-coupled device (ICCD) camera is an advanced imaging device that combines the capabilities of both an image intensifier and a charge-coupled device (CCD) sensor to achieve...Photonics DictionaryMediaNew ZL41 Cell & Sona sCMOS - Easy, Flexible, Cost-Effective Super Resolution Microscopy - Andor TechnologyEasy and Cost-Effective Super-Resolution to ~100 nm. Andor has introduced two new highly innovative sCMOS cameras, both delivering easy and cost-effective access to super-resolution capabilities and...Photonics.com 11/20/2023Supporting the Photonics Industry from the Laboratory - LIGHT MATTERS 01.2018On this month's episode of Light Matters, we take you to central Massachusetts as Worcester Polytechnic Institute and Quinsigamond Community College take on a comprehensive joint photonics laboratory...Photonics.com 1/18/2018pco - Frequency Domain Fluorescence Lifetime Imaging Microscopy - A Hands-On SessionThis hands-on session about FD-FLIM by Gerhard Holst from PCO AG was given as part of the FLIM Symposium "Beginners Guide to Fluorescence Lifetime Imaging in Life Sciences".Photonics.com 10/12/2021iSpectrophotometer & Spray-On Lens - LIGHT MATTERS 05.29.2013-Spray-on Lens -iSpectrophotometer -Polariton Laser This week on Light Matters: a spray-on flat lens works in the ultraviolet, a smartphone camera becomes a high-resolution spectrophotometer, and a...Photonics.com 5/29/2013Photonics Buyers' Guide CategoriesPicosecond LasersAutomated MicroscopesScanning Electron Microscopes (SEM)A scanning electron microscope is an electron microscope that uses a beam of electrons -- accelerated to high energy and focused on the sample -- to scan the sample surface, ejecting secondary...Research MicroscopesCompaniesMad City Labs Inc.Mad City Labs, Inc. is a leading manufacturer of piezo nanopositioning systems and instrumentation capable of sub-nanometer positioning resolution. Our product line of nanopositioners,...Photonics Buyers' GuideAndor TechnologyAndor Technology Ltd is a global developer and manufacturer of high-performance scientific cameras, microscopy systems and spectrographs for academic, government, and industrial applications. Founded...Photonics Buyers' GuideAllied Vision Technologies GmbHFor over 30 years, Allied Vision has helped people see the bigger picture to achieve their goals. We believe knowledge is power and always strive to deliver valuable insight with our technology...Photonics Buyers' GuidePrior Scientific Inc.For over 100 years, Prior Scientific has been manufacturing precision optical and electro-mechanical equipment and instrumentation. Prior is a leader in the development of precision motion,...Photonics Buyers' Guide