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Nonconfocal 3D Microscopy Combines Real-Time Images with Depth of Focus

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The technique holds the promise of greater accuracy and efficiency for neuroscience, vascular research, and cytopathology.

GARY GREENBERG, HNU-EDGE LLC

Stereo 3D microscopes produce real-time 3D images, but they are usually limited to low-magnification applications, such as dissection. Most compound light microscopes produce flat, 2D images because high-magnification microscope lenses have inherently shallow depth of field, rendering most of the image out of focus. Because of the shallow depth of focus of microscopes, microscopists usually prepare 5- to 10-µm sections of their samples to eliminate the problem of out-of-focus blur. This is problematic because the average cell is between 10 and 30 µm. Examining 5- to...Read full article

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