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microscope Dictionary Terms

scanning tunneling microscope
A high-resolution imaging instrument that can detect and measure the positions of individual atoms on the surface of a material. A very fine conductive probe is placed at a distance of 10 to 20 Å above the surface of a conductive sample, and a...
image intensifier
An image intensifier, also known as an image intensification tube or image intensification device, is a specialized...
atomic force microscope
An atomic force microscope (AFM) is a high-resolution imaging and measurement instrument used in nanotechnology, materials...
Greenough microscope
A form of a stereoscopic microscope having paired objectives, prisms and eyepieces, and invented by H. Greenough.
microscope immersion fluid
The liquid used in microscopy to fill the space between the high-power objective lens and the microscope slide in order to...
microscopic
Characteristic of an object so small in size or so fine in structure that it cannot be seen by the unaided eye. A...
compound microscope
projection x-ray microscope
A microscope that uses an extremely fine x-ray focal point to produce an enlarged photographic image of a sample. Also known...
video-intensified microscopy
The observation of specimens by a microscope linked to a television camera and an image processor.
piezoelectric motion systems
Piezoelectric motion systems are mechanical systems that utilize piezoelectric materials to generate controlled motion or...
photon tunneling microscope
An instrument in which visible light beyond the critical angle from a metallurgical microscope is focused on a reference...
microscope stage
The component of a microscope on which the sample or slide to be examined is placed. Depending on the design of the...
fluorescent screen
A fluorescent screen refers to a phosphorescent or fluorescent-coated surface that emits visible light when exposed to other...
power
With respect to a lens, the reciprocal of its focal length. The term power, as applied to a telescope or microscope, often...
stereomicroscope
Wide-field, low-magnification device that consists of two compound microscopes focused on a single object, producing an...
stereomicroscopy
The use of a specialized optical microscope designed to provide a more three-dimensional view of a sample. Stereomicroscopy...
high-voltage electron microscope
An imaging device whose technology contributes three specific advantages: ability to study large solid specimens that...
dark-field illumination
The transmission of light by a condenser to observe either very small particles or very fine lines with a microscope.
cardioid condenser
An oil immersion condenser used to permit only light that has been diffracted or dispersed by a microscope specimen to enter...
point-probing scanning optical microscope
modulation transfer function analysis/measurement equipment
MTF analysis/measurement equipment refers to instruments and tools used to measure and analyze the modulation transfer...
indirect illumination
The light formed by visible radiation that, in traveling from light source to object, undergoes one or more reflections. In...
Bertrand lens
A small convergent lens, located between the objective and eyepiece of a microscope, that focuses an image of the upper...
ultraviolet microscopy
The study and photographing of microscope specimens in ultraviolet light; using an optical microscope containing fluorite...
electron microscope
A device utilizing an electron beam for the observation and recording of submicroscopic samples with the aid of photographic...
field ion microscope
An extremely powerful microscope that renders individual ionized atoms visible by using an electric field to propel the ions...
microscope objective
The lens located at the object end of a microscope tube. A wide range of objectives is available, including simple...
objective
The optical element that receives light from the object and forms the first or primary image in telescopes and microscopes....
aperture diaphragm
The second adjustable iris diaphragm in an optical system. In the common microscope condenser system, it usually is located...
low-coherence interference microscope
An interference microscope that uses a light beam originating from a low-coherence light source. The sample is placed in one...
traveling microscope
A measuring instrument composed of a microscope and reticle, and mounted on a calibrated slide mechanism. May be used...
cinemicrography
Cinematography performed with the use of a microscope to film the actions of microscopic specimens.
virtual histology
Virtual histology refers to the use of digital technology and computational methods to simulate or recreate histological...
x-ray shadow microscope
microphotometer
An instrument capable of measuring the transmitted or reflected luminance from a very small area seen under a microscope....
metallographic polishing machine
A small optical polishing machine intended for polishing the surface of a metal specimen before etching for examination...
superresolution
Superresolution refers to the enhancement or improvement of the spatial resolution beyond the conventional limits imposed by...
lens
A lens is a transparent optical device that focuses or diverges light, allowing it to pass through and form an image. Lenses...
scanning probe microscope
See atomic force microscope; magnetic force microscope; near-field scanning optical microscope; scanning tunneling...
flying spot microscope
A microscope that uses a flying spot scanner, directed through the eyepiece, as a light source to determine the features of...
gram negative
Gram-negative bacteria are a group of bacteria that possess a cell envelope composed of a thin layer of peptidoglycan...
micrurgy
The use of a micromanipulator in combination with a microscope for the purposes of examining, dissecting, or the...
comparison microscope
Two microscopes that are coupled on a common stand, the two images being projected side by side in the field of view of a...
calcite interference microscope
A microscope that allows examination of a small crystal and conveniently provides linearly polarized object and reference...
focus
1. The focal point. 2. To adjust the eyepiece or objective of a telescope so that the image is clearly seen by the observer....
electron micrograph
The photographic recording of images produced by the electrons from an electron microscope. The electron beam carries the...
confocal scanning microscope
A microscope design that involves apertures inserted in conjugate plane positions inside the microscope, with one aperture...
laser microscopy
Technique using functional optical microscope with the addition of a coherent source collinear with the image path. The...
aplanatic surface
An aplanatic surface is an optical surface that is specifically designed or shaped to minimize spherical aberration and...
electric quadrupole lens
A device that uses four electrodes set in an alternating positive-negative polarity series to focus the beams of charged...
microspectrophotometer
A specialized spectrophotometer for use through a microscope on very small areas of an object.
color-translating microscope
A type of compound microscope that uses three visible wavelengths to translate details produced by invisible radiation.
coordinate measuring microscope
An instrument used to measure the coordinates of a point on an object such as a photographic plate.
aspheric mirror
An aspheric mirror is an optical mirror surface that deviates from the shape of a perfect sphere, having a non-spherical...
brightfield
Brightfield refers to a type of microscopy and imaging technique in which the specimen is illuminated with a white light...
x-ray microprobe analysis
The method of acquiring characteristic x-ray spectra from microscopic samples by use of the combination of a scanning...
microscope, electron
ultramicroscope
A dark-field microscope used to view extremely small objects. These objects are suspended in a gas or liquid in an enclosure...
microtome
A device used for slicing very thin specimens in preparation for mounting on a microscope slide.
retardation
The phase change of one of the two split beams of an interference microscope.
eyepiece
Also known as ocular. The lens system used between the final real image in a visual optical system and eye. It acts as an...
Senarmont compensator
A type of compensator for use with a microscope and consisting of a quarter-wave plate in a fixed position and a rotatable...
microscope eyepiece
An eyepiece located at the near end of the microscope tube. It often is a simple Huygens eyepiece, but compensating and...
fluorescent microscope
A type of optical microscope that allows the specimen being viewed to be irradiated by ultraviolet, violet and occasionally...
autostigmatic microscope
A microscope to which a beamsplitter and illuminated reticle have been added to measure the radius of curvature of a...
micrograph
A graphic reproduction of an object formed by a microscope or another optical system. Also an instrument used to make tiny...
infinity space
In a microscope, a space reserved to accommodate an optical filter or polarizer.
optical bench
A support for optical parts comprising a solid bed that permits precise longitudinal movement of one component relative to...
photomicrographic camera
A still or motion-picture camera designed to photograph through a microscope. Photomicrographic equipment usually contains a...
voltage contrast analysis
A nondestructive testing method for very large scale integration circuits, using a scanning electron microscope to monitor...
achromat
An achromat, in the context of optics, refers to a type of lens or lens system designed to reduce chromatic aberration....
scanning acoustic microscope
Also called scanning laser acoustic microscope. A device that uses high-frequency ultrasound waves to penetrate surfaces. A...
eye test apparatus
Instruments used by ophthalmologists and optometrists to study the eye. There are, for instance, the ophthalmoscope to...
comparator
1. An eyepiece or magnifier with a scale at its image plane. That scale is placed in contact with an object, permitting...
point spread function
The point spread function (PSF) is a fundamental concept in imaging that describes the response of an imaging system to a...
aspheric lens
An aspheric lens is a type of lens whose surface profiles deviate from the traditional spherical shape. Unlike spherical...
lens blank
A lens blank is a piece of optical material in a raw, unfinished state, typically in the form of a disk or block, from which...
microspectroscope
A system composed of a prism spectroscope and compound microscope to provide the visual comparison of two spectra...
multiple wavelength interferometry
A specific form of phase shifting interferometry - commonly referred to as multiple wavelength phase shifting interferometry...
field of view
The field of view (FOV) refers to the extent of the observable world or the visible area that can be seen at any given...
eikonometer
A scale attached to a microscope eyepiece that is seen superimposed on the image and that is used to measure the dimensions...
grain isolating diaphragm
In a microscope, the diaphragm located between the Bertrand lens and the eyepiece, which is closed during conoscopic viewing...
illuminated table
A desklike apparatus with an opal glass surface illuminated from beneath by fluorescent tubes. It is equipped with roll...
microcrystal
A microscopic crystal found in an intricately crystallized substance that is only visible under a microscope.
prism
A prism is a geometric optical element with flat, polished surfaces that refract light. It is typically a solid, transparent...
conoscope
An optical instrument, generally a polarizing microscope, that is used to determine the interference figures and optical...
wide-field fluorescence microscopy
Wide-field fluorescence microscopy uses either naturally occurring structures or staining with fluorescent tags that are...
full wave compensator
A piece of uniform birefringent material placed at a 45° angle to the plane of polarization in a polarizing microscope...
concave and convex spherical mirror
Concave and convex spherical mirrors are types of curved mirrors that have surfaces shaped like segments of spheres. These...
television microscope
A device designed to enlarge the image of a microscopic object by television process. It may be a flying spot scanner that...
Amici objective
A form of high-power microscope objective.
reticle
A reticle, also known as a reticule or graticule, is a pattern or set of markings placed in the focal plane of an optical...
instrument myopia
The tendency to adjust an instrument such as a microscope so that the viewed image appears much closer than infinity.
multi-axis alignment
Multi-axis alignment refers to the process of aligning or adjusting components or systems in multiple spatial dimensions or...
microscopy
Microscopy is a scientific technique that involves the use of microscopes to observe and study objects that are too small to...
mesoscope
In the field of optics and imaging, a mesoscope refers to an optical instrument that is designed to observe and capture...
petrographic specimen preparation
The grinding and polishing of rock samples, to a thickness of less than 0.05 mm, for study with petrographic microscopes....
metallography
The analysis of metal structure using an optical or electron microscope, generally with a camera, to record observations.
inverted microscope
A microscope designed so that the specimen is located face down above the objective.
Smith-Baker microscope
A transmission interference microscope that produces interference patterns of a sample by using birefringent plates that...
metallographic microscope
A specially designed microscope for observing the etched surface of a polished metal specimen. The specimen is often laid...
Abbe illumination
Image of a uniform source through the sample of a microscope image system. Light from the sample plane is reimaged by the...
reflecting microscope
A microscope that uses a reflecting objective; often used with ultraviolet or infrared radiation.
phase contrast microscopy
Phase contrast microscopy is an optical microscopy technique that enhances the contrast of transparent and colorless...
binocular microscope
A microscope designed with two eyepieces (oculars), permitting the use of both eyes when viewing through the instrument.
Brucke loupe
A telemicroscope in which a negative eyepiece is used to produce erect images.
magnetic force microscope
A variation of the atomic force microscope that operates by scanning a tiny ferromagnetic probe (or a magnetized tip) over a...
microstereoscope
A binocular microscope designed for the viewing of stereo pairs. As these stereoscopic image pairs are seen through a...
measuring eyepiece
Also known as an eyepiece micrometer. A microscope eyepiece that has a finely divided scale ruled or photographed on a...
image optics
Any form of lens, optical system (camera, telescope, microscope, etc.), or opto-electronics utilized for the purpose of...
Becke apertometer
Device used to measure the numerical aperture of a microscope, composed of a 14-mm-thick glass block with numerical aperture...
laser confocal microscope
ultraviolet photomicrography
The photographic recording that uses ultraviolet radiation to irradiate the microscope sample being examined and to form an...
interphako interference microscopy
Measures the refractive indices axially from the fiber profile. Microscopy technique provides an interferogram with high...
transmission electron microscope
A transmission electron microscope (TEM) is a powerful microscopy technique that uses a beam of electrons to create...
ophthalmic instruments
A family of specialized instruments used by ophthalmologists and optometrists to study a patient's eyes and prescribe...
micromanipulator
A device that provides means for accurately moving minuscule tools over the surface of a microscopic object. The motion...
nodal bench
A bench with instrumentation including a collimator, a microscope, positioners and a nodal slide used to rotate a lens about...
spheric lens
A spheric lens, also known as a spherical lens, is a type of optical lens with at least one surface that is part of a...
phase annulus
A term for the ring-shaped stop in a phase contrast microscope. The phase annulus limits the amount of light that reaches...
histochemical staining
Histochemical staining is a laboratory technique used in biology and medicine to visualize specific chemical components...
dry objective
A microscope objective designed to be used without liquid between the cover glass and the objective, or, in the case of...
single molecule localization microscopy
Single molecule localization microscopy (SMLM) is a group of super-resolution microscopy techniques that surpass the...
gram positive
Gram-positive bacteria are a group of bacteria that have a thick cell wall composed primarily of a substance called...
near-field scanning optical microscope
A scanning probe microscope that analyzes the surface of a specimen by recording the intensity of light as it is focused...
optical dynameter
A small low-power microscope or magnifier with a scale that is used to measure the exit pupil diameter and eye relief on...
knife-edge scanning microscope
An imaging device originally created to image whole mouse brain volumes at microscopic resolution. The main component of the...
electron-gun system
An electron-gun system is an assembly used to generate and control a focused beam of electrons. Electron guns find...
direct illumination
Light produced by visible radiation that moves from the light source to the object without reflection. With respect to...
mechanical tube length
In a microscope, the physical distance between the focal points of the objective lens and the eyepiece. The standard tube...
infrared microscope
A type of microscope that uses radiation in the infrared region to illuminate objects that are opaque to visible radiation....
whole slide imaging
Whole slide imaging (WSI), also known as virtual slide microscopy or digital pathology, is a technology that involves the...
spectrum measuring instrument
A traveling microscope or an automatic microdensitometer used to measure the spectrum plate obtained in a spectrograph.
optical cements and adhesive
Optical cements and adhesives are specialized materials used in the assembly and bonding of optical components in optical...
phase-contrast microscope
A microscope that has an annular stop in the lower focal plane of the condenser, and a quarter-wave retarding and absorbing...
antireflection coating
An antireflection coating (AR coating) is a thin film or coating applied to optical surfaces, such as lenses or windows, to...
Koehler illumination
A two-stage illuminating system for a microscope in which the source is imaged in the aperture of the substage condenser by...
petrographic microscope
A microscope equipped with a polarizer, an analyzer and a Bertrand lens to focus on the upper focal plane of the objective....
Linnik interference microscope
A Michelson-type interference microscope used to produce interference patterns of reflective specimens through the...
microscope
An instrument consisting essentially of a tube 160 mm long, with an objective lens at the distant end and an eyepiece at the...
dichroscopic eyepiece
An eyepiece used in a polariscope or polarizing microscope to give a comparison view of the same object under illumination...
quadrupole lens
A device used in electron microscopes and particle accelerators to focus electron beams by the arrangement of four...
scanning electron micrograph
The picture formed by the scanning beam of electrons in a scanning electron microscope.
field emission microscope
An image-forming instrument in which a strong electrostatic field causes cold emission of electrons from a sharply rounded...
dust counting microscope
A microscope that has been modified to permit the quantitative analysis of dust samples.
scanning electron microscope
A scanning electron microscope (SEM) is a powerful imaging instrument used in scientific research, materials...
split-image microscope
A mask-alignment microscope used to produce and inspect microcircuits in the electronics industry. It provides flat-field,...
reflecting objective
An image-forming system that uses mirrors rather than lenses. Such objectives are frequently used for astronomical...
lensless microscopy
Lensless microscopy, also known as computational or holographic microscopy, is an imaging technique that captures and...
histology
Histology is the branch of biology and medicine that involves the study of the microscopic structure of tissues and organs...
radiuscope
A microscope to which a beamsplitter and illuminated reticle have been added that is used to measure contact lenses.
emission microscope
A type of electron microscope in which the specimen also serves as the cathode source.
optical assembly
An optical assembly refers to a collection of optical components that are carefully arranged and aligned to perform a...
chromatic aberration
Chromatic aberration is an optical phenomenon that occurs when different wavelengths (colors) of light are refracted by a...
centrifuge microscope
A microscope that can be used to observe and magnify microscope specimens while they are being centrifuged. The objective...
Abbe condenser
A two-lens arrangement intended to image light into a microscope slide sample. The primary aberrations present are red and...
nuclear track emulsion
A photographic emulsion of the silver-halide type that is used to record the path of a charged traveling particle. The...
interference microscope
A special form of microscope that utilizes interference for observing and measuring the phase and optical thickness in...
mirror blank
A mirror blank refers to the initial piece of material from which a mirror is made. It is typically a flat or slightly...
ultraviolet lens
A microscope used either to detect selective absorption of various wavelengths by the specimen or to achieve increased...
optical testing instrument
An optical testing instrument is a device or system used to evaluate and measure the performance, quality, and...
photomicrography
The use of a microscope in photographing objects. A device for photomicrography includes a light source, microscope and...
diffraction rings
1. The ring patterns of light that seem to encircle particles in the field of a microscope. 2. See Newton's rings.
Becke line
A band of light that appears along the outer edge of a transparent material under microscopic investigation and that moves...
substage condenser
In a microscope, the optical assembly that focuses light on the specimen and into the objective.
stage micrometer
In microscopy, a calibrated scale on a slide that may be viewed to determine the exact magnification factor of the...
pinhole eyepiece
A type of eyepiece, or the modification of an eyepiece, in which a small hole, without a lens, functions as the eye lens;...
oil-immersion objective
A form of high-power microscope objective where the space between the object and the first element is filled with an oil...
microphotometry
The measurement of the intensity of spectral lines by the examination of a very small area under a microscope and the...
fiber-based confocal luminescence microscope
A microscope in which laser light is delivered through single-mode fibers that replace the pinhole usually used in confocal...
optics
Optics is the branch of physics that studies the behavior and properties of light, including its interactions with matter...
compensating eyepiece
A microscope eyepiece designed for use with apochromatic objectives. Since apochromatic objectives are undercorrected for...
counting chamber
In microscopy, the chamber that is contained on a microscope slide to hold a certain amount of fluid. It is calibrated...

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